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KMID : 0364820230590010001
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Korean Journal of Microbiology
2023 Volume.59 No. 1 p.1 ~ p.7
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Biochemical characterization of a PadR-type transcriptional regulator CmmRII in Streptomyces sp. SJ1-7
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Choi Um-Ji
Tsevelkhoroloo Maral
Chi Won-Jae
Hong Soon-Kwang
Lee Chang-Ro
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Abstract
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Chromomycin A3 is an aureolic acid type antibacterial and antitumor compound produced by Streptomyces species. The biosynthetic gene cluster of chromomycin A3 is composed of more than 30 genes involved in polyketide biosynthesis and modification, sugar biosynthesis and transfer, and resistance, but the regulatory mechanisms of their expression are poorly understood. In this study, we analyzed biochemical features of the AfsR-type transcriptional regulator CmmRI and PadR-type transcriptional regulator CmmRII, which are located within the biosynthetic gene cluster of chromomycin A3 of Steptomyces sp. SJ1-7, isolated from soil surrounding the root of a Pinus densiflora plant in Sangju, South Korea. CmmRII interacted with several putative promoter regions of chromomycin A3 biosynthesis genes, whereas CmmRI interacted with no promoters tested. The presence of chromomycin A3 hardly affected the interaction between CmmRII and the promoter region. Purified CmmRII existed in as a monomeric form. These results imply that CmmRII is an important transcriptional factor in expression regulation of the biosynthetic gene cluster of chromomycin A3 in Steptomyces sp. SJ1-7.
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KEYWORD
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Streptomyces, chromomycin A3, gene expression, transcriptional regulator
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